SECTION 1

LABORATORY PROCEDURES

1. The documentary system

2. Culture media techniques

3. IVF incubator handling

4. Preparation for follicle aspiration and isolation of cumulus-oocyte-complexs(COCs)

5. Semen collection and sample reception

6. Spermiogramm , Makler chamber ,Neubaueur chamber, CASA

7. Sperm viability testing

8. Sperm preparation

9. Sperm selection using hyaluronan binding

10. IVF—Insemination

11. ICSI

12. Oocyte invitro maturation --- Isolation of COCs and culture for maturation

13. Artificial Oocyte Activation

14. Cryopreservation—Sperm TESA and MESA

15. Vitrification—Oocytes,2PN,Embryos,Blastocysts

16. Slow freezing

17:Oocyte selection

18: Zygote selection

19: selection and grading of embryos on Day 23 and Blastocyst on Day 56

20: Time lapse imaging

21: Assisted hatching

22: Zona manipulation at various embryo stages : Mechanichal Acidic medium

23: Biopsy : Polar body , Blastomere , Trophectoderm

24: Sampling of cells after biopsy for a CGH

25: Embryo transfer---Standard and ultrasound guided

26: Hyginic standards in IVF laboratory

27: Quality control of IVF laboratory supplies—MEA

28: Trouble shooting in IVF laboratory

29: Microscopy

30: Fertility preservation

1. Ultrasound evaluation in treatment management

2. 3D Ultrasound and Diagnosis of utrine anomalies

3. Infertility ultrasound evaluation

4. Office hysteroscopy

5. Hysteroscopic management of uterine septum

6. Hysteroscopic lysis of intrauterine adhesions

7. Hysteroscopic management of submucosal fibroids

8. Saline infusion sonohysterography

9. General principles of laparoscopy

10. Microlaparoscopy

11. Laparoscopic myomectomy and minilaparotomy

12. Laparoscopic excision of large endometrioma

13. Laparoscopic management of Subtle fimbrial pathology secondry to endometriosis

14. Laparoscopic management of mature cystic teratoma

15. Tubal canulation for proximal tubal disease

16. Unexplained infertility

17. Male evaluation for infertility

18. Hysterosalpingography

19. Intrauterine insemination: clinical perspective

20. GnRH Agonist to trigger final follicular maturation

21:GnRH Agonist protocols

22: GnRH Agonists before IVF for Advanced Endometriosis

23: Microdose GnRH Agonist flare protocol

24: Gonadotropin-Releasing Hormone Antagonist cycle for IVF

25: Minimal stimulation for IVF

26: Ovarian Hyperstimulation Syndrome (OHSS)

27: The prediction of OHSS

28: Tips on technique of Embryo transfer

29: Difficult Embryo transfer

30: IVM for fertility treatment and fertility preservation

31: Complications and outcome of IVF

32: Obstetric complications after IVF

33: Twins pregnancy after ART

34: IVF and Endometriosis

35: Leiomyoma of the uterus

36: PCO and its management

37: Adenomyosis ; imaging and treatment

38: Ectopic pregnancy : Evaluation and Management

Introduction to In vitro fertilisation (IVF), Andrology, IVF, cryopreservation lab, equipments and structure

Hands on semen analysis, semen processing, sperm cryopreservation

Hands On Semen Preparation for intrauterine insemination (IUI) and intracytoplasmic sperm injection (ICSI)

Hands On Egg pick up, its protocol and trouble shoots

Hands on culture dish preparation

Gamete handling and insemination in embryology lab

Hands on catheter loading of embryos and transfer using non gamete cells. Luteal support

Obstetrics and Gynecology Consultant

Professor of Human Genetics and IVF MUST University

IVF lab director, Hawaa Fertility Center

MD in microbiology

PH.D. in microbiology

ESHRE membersheip

ASRM membership